Characterization of the digestive-tract microbiota of Hirudo orientalis, a european medicinal leech.
نویسندگان
چکیده
FDA-approved, postoperative use of leeches can lead to bacterial infections. In this study, we used culture-dependent and culture-independent approaches to characterize the digestive-tract microbiota of Hirudo orientalis. Surprisingly, two Aeromonas species, A. veronii and A. jandaei, were cultured. Uncultured Rikenella-like bacteria were most similar to isolates from Hirudo verbana.
منابع مشابه
Characterization of the Digestive Tract Microbiota of Hirudo orientalis ( Medicinal Leech ) and Antibiotic Resistance Profile SPECIAL TOPIC
408e Although blood letting and the use of leeches dates back to pharaonic Egypt,1 the U.S. Food and Drug Administration only approved the use of Hirudo medicinalis as a medical device in 2004.2 Leeches are used by plastic,3–7 maxillofacial,8–10 and other surgeons11,12 to aid salvage of venously congested pedicled flaps,13,14 freetissue transfers,3,15–17 replanted digits,6,18–25 ears,26–32 lips...
متن کاملHost Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential
Digestive-tract microbiota exert tremendous influence over host health. Host-symbiont model systems are studied to investigate how symbioses are initiated and maintained, as well as to identify host processes affected by resident microbiota. The medicinal leech, Hirudo verbana, is an excellent model to address such questions owing to a microbiome that is consistently dominated by two species, A...
متن کاملCulture-independent characterization of the digestive-tract microbiota of the medicinal leech reveals a tripartite symbiosis.
Culture-based studies of the microbial community within the gut of the medicinal leech have typically been focused on various Aeromonas species, which were believed to be the sole symbiont of the leech digestive tract. In this study, analysis of 16S rRNA gene clone libraries confirmed the presence of Aeromonas veronii and revealed a second symbiont, clone PW3, a novel member of the Rikenellacea...
متن کاملPredation in two species of leech under laboratory conditions
Two 60 day experiments on predation behavior of leeches were conducted concurrently. In the first, medicinal leeches, Hirudo orientalis (Utevsky and Trontelj, 2005) were fed to satiation on bovine blood and subsequently transferred into two large opaque plastic containers of equal size but with differing temperatures, 6-7?C and 23-24?C, containing starved Erpobdella octoculata (L.). At the high...
متن کاملMetagenomic analysis of the medicinal leech gut microbiota
There are trillions of microbes found throughout the human body and they exceed the number of eukaryotic cells by 10-fold. Metagenomic studies have revealed that the majority of these microbes are found within the gut, playing an important role in the host's digestion and nutrition. The complexity of the animal digestive tract, unculturable microbes, and the lack of genetic tools for most cultu...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 74 19 شماره
صفحات -
تاریخ انتشار 2008